Applied techniques at a glance

Molecular and microbiological techniques
Isolating of genomic DNA from announced modelorganisms
PCR, Reverse transcriptase- and inverse PCR
Cloning and subcloning (D.discoideum)
Cultivating of E. coli
Mini-, Midi-, Maxipreparation
Gelelektrophoresis
Isolating a fragment from a DNA - gel
Measuring the concentrations of DNA and RNA
Digestion with restriction enzymes
Ligation
Transformation of E.coli and D. discoideum via heatshock and electroporation
Characterising of recombinant clones
Northern Blot and Developmental Northern Blot
Construction of (x-ray) marked probes (DNA and RNA) for Blot research
Sequencing with x-ray as well as fluorescence (Li-cor) dideoxy method
Isolating of RNA with BEADS and working with RNA

Microscopic techniques
Light microscope
Fluorescence microscope
Confocal Laser Scanning microscope (CLSM)
Transmission microscope
Raster electron microscope
Video microscopie

Proteinbiochemistry techniques
Induction of Protein expression in E.coli
Preparation of proteins
Dialysis
Stripping of Proteins
SDS – PAGE
Commassie staining of SDS gel
Bradford Assay
Cook Assay
Measuring of fluorescence polarisation
Biacore linkage studies
Fast Protein Liquid Chromatography (FPLC)

Immunological techniques
Western Blot
Immunostaining

Other techniques
Phagocytosis Assay (D.discoideum)
Different staining techniques i.e. Calcufluor staining